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Biochemistry

Biochemistry

628 quality and many readers will be surprised by the varying emphasis placed on the different areas as well as their clarity. It is untbrtunate, for ...

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628 quality and many readers will be surprised by the varying emphasis placed on the different areas as well as their clarity. It is untbrtunate, for example, that the results of the many transgenies experiments carried out in domestic animal have not been reported in this book. It many also be considered surprising that only one genetic map has been published. Molecular genetics is a rapidly moving field and information such as, for example, the list of the known RFLPs in various domestic species is already out of date. It is also regrettable that the length polymorphisms of genomic (CA) repeats, the so-called microsatellites, which have been reported to constitute an interesting source of polymorphisms both in man and mouse and which ,are abundant in domestic animals, have not been considered. In spite of the above, this book contains some interesting contributions which, on their own would justify a copy being bought for the shelves of agricultural and veterinary school libraries. The chapter dealing with repetitive DNA sequences and that on DNA sequencing, for example, are both of great didactic value. JL Gu6net

Biochemistry, edited by Donald Voet and Judith G Voet, John Wiley and Sons, 1990, XVII + 1223 p, £ 23.50 Many Biochemistry text books exist, however, some are more often quoted than others, for example, to ask for the Lehninger will be understood in all laboratories working in the field of Biochemistry. Why other text books, and who would dare challenge this well established first position? Biochemistry, by Donald G Voet from the University of Pennsylvania, is obviously a challenger, and an excellent one. The presentation and illustrations of the book are excellent, and the use of color, in quadrichromy throughout the book gives ai-, esthetical impression of the field; this will certainly please students and help them believe that biochemistry is a worthwhile science. All important fields of biochemistry are presented in this book, from small molecules to macromolecules, from intermediary me:abolic pathways to the monomolecule biosynthesizing machinery, or from the cell envelope to electron transfer. If you chose to study this latter part of (complex) biochemistry, for example, you would choose chapter 20, choose Electron transl~ort and oxidative phosphorylation, or chapter 22, photosynthesis, and learn the complex organisation of the electron transfer chain. A short bibliography (ending in 1989) of appropriate references is also available. Upon reading the book, it was very difficult to find any weak points: the only one I found - and which is still present in many specialized as well as text books, is that the origin of life is described without questioning the universally admitted (but probably wrong) model ot Lie prebiotic soup... Just to say that this treaty is almost perfect! AL Lecocq

M o d e r n T h i n - L a y e r C h r o m a t o g r a p h y (Chromatographic Science Series/52), edited by N Grinberg, Marcel Dekker, 1990, pp 504, $ 99.75 (US and Canada), $ 119.50 (all other countries)

Today chromatography plays a very important role among analytical techniques. Every preparative working chemist and biochemist knows that it is almost impossible to imagine a laboratory without chromatographic equipment. Chromatography is not only used in basic research but also in industry and is at present the outstanding method for separating organic and biological mixtures. In liquid chromatography (LC), there are 2 ways to operate: column and open-bed or thin-layer chromatography (TLC), the lattc~ being the subject of Modern T/finLayer Chromatography, edited by N Grinberg (Marcel Dekker Inc, USA). This book was published as part as a series of monographs (Vol 52) dealing with chromatographic science. The topicality of this type of chromatography is underlined by the fact that it is already the third contribution to TLC techniques within this series. The book is divided into l0 chapters written by 6 authors. After the introduction, the second chapter gives a derailed survey of all kinds of stationary phases commonly used in thinlayer chromatography. A chapter about the mobile phases in TLC follows. In the fourth chapter, the theoretical aspects of TLC are described; and chemists are given an in-depth account of chromatographic phenomena. Discussions about the possibility of quantitation in TLC and instrumentation follow in the next chapters. The most important part of the book is chapter 7: it deals with special techaiques that may improve utilization and separation, for example centrifugal layer chromatography (p 331) and chiral separations (p 398). This chapter is subdivided into contributions on continuous TLC, forced-flow TLC, bidimensional TLC, gradients in TLC, chiral separation in TLC and preparative TLC. It re, dews nearly all the modem developments in TLC. Chapters 8 and 9 present a discussion on mobile phase and time optimization and the relationship between thin-layer and column chromatography, respectively. A contribution

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chapter contains several references from tl~e original literature (in total i 506 references) making this book a useful reference guide. Obviously, this book is addressed to all chemists and biochemists, as well as to students in these disciplines, especially in analytical chemistry, who deal with TLC and who wish to improve their understanding of separation phenomena. Finally, I can recommend it to everyone who uses TLC techniques as it will help these individuals to use them more effectively; and I hope that this book will find its place in every chemistry faculty library.

C Meier