Expression of embryonic lethal abnormal vision-like protein HuR associated with cyclooxygenase-2 in oral squamous cell carcinoma

Expression of embryonic lethal abnormal vision-like protein HuR associated with cyclooxygenase-2 in oral squamous cell carcinoma

542 Free Papers—Poster Presentations the left buccal submucosal region. Case-2 patient presented with a complaint of a rapidly growing painless swelli...

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542 Free Papers—Poster Presentations the left buccal submucosal region. Case-2 patient presented with a complaint of a rapidly growing painless swelling in the left buccal submucosal region. 2. Course until first visit: Case-1 patient was referred to us by his general practitioner [a nearby physician] for suspected left parotid gland tumour. Case-2 patient was referred to us by his general practitioner [a nearby physician] for suspected left maxillodental infection. 3. Clinical findings and diagnosis at the first visit: Case-1 patient had a movable mass with well-defined borders, which was relatively elastic, soft and not tender on palpation, without interfering with opening his mouth. The clinical diagnosis at first visit was lipoma of the buccal submucosa. Case-2 patient had a bulky movable elastic soft painless mass in the shallow region of the buccal submucosa. The clinical diagnosis at first visit was pleomorphic adenoma of the buccal submucosa. 4. Computed tomography and magnetic resonance imaging findings: Case-1 and Case-2 patients both had a mass lesion with well-defined borders. Both patients had no abnormal finding in the surrounding mucosa of their tumour and no malignant features such as bone destruction of the maxilla or mandible. 5. Histopathological findings: Case-1 and Case-2 patients both had a non-Hodgkin’s lymphoma. doi:10.1016/j.ijom.2009.03.507

P35 A new method to establish cell line Tca8113/CBP by inducing multi-drug resistance to carboplatin in mice model of human tongue squamous cell carcinoma F. Ge ∗ , D.Z. Wang, J. Hu Department of Oral and Maxillofacial Surgery, West China Stomatology College of Sichuan University, China

Background and Objectives: Multi-drug resistance (MDR) in human head and neck squamous cell carcinoma (HNSCC) constitutes a major obstacle to the effectiveness of chemotherapy. In previous studies MDR was mostly induced in vitro. We report a novel in vivo method of inducing MDR in nude mice with xenotransplanted Tca8113 cells. Methods: We injected carboplatin, a chemotherapeutic agent used to treat HNSCC, around the tumours for 10 weeks. Subsequent cell survival assay of dissociated tumour cells suggested that MDR had been successfully induced.

Results: A subsequent cell survival assay of dissociated tumour cells suggested that MDR had been induced successfully. Immunocytochemistry, reverse transcription polymerase chain reaction and Western blot analysis showed that the expression levels of MDR-related proteins, including topoisomerase II, multidrug resistance-associated protein and glutathione transferase, were elevated in the induction group. Conclusions: The study concluded that in vivo induction of MDR provides a useful method for the establishment of animal models of MDR. doi:10.1016/j.ijom.2009.03.508

P36 Expression of embryonic lethal abnormal vision-like protein HuR associated with cyclooxygenase-2 in oral squamous cell carcinoma J.D. Cha 1,∗ , S.J. Li 1 , J. Kim 1,2 , I.H. Cha 1,3 1 Oral Cancer Research Institute, Yonsei University, South Korea 2 Department of Oral Pathology College of Dentistry, Yonsei University, South Korea 3 Department of Oral and Maxillofacial Surgery, College of Dentistry, Yonsei University, South Korea

Background and Objectives: The human embryonic lethal abnormal vision (ELAV)like protein HuR is involved in the stabilisation of the mRNAs by binding to AU-rich elements in the 3’ untranslated region of the mRNA of a group of genes implicated in the regulation of cellular growth, angiogenesis, and rapid inflammatory response. Increased cytoplasmic HuR expression occurs in several cancer types, where it may contribute to the increased cyclooxygenase-2 (COX- 2) expression observed during tumourigenesis and metastasis. The aim of this study was to investigate the correlation between COX-2 and HuR in oral squamous cell carcinoma (OSCC). Methods: The expression patterns for HuR and COX-2 were assessed via immunoblotting of OSCC cell lines treated with HuR small interfering RNA and immunohistochemical analysis of 105 OSCC samples. Results: In tumour tissues, nuclear HuR expression was observed in 91.67% cases and cytoplasmic expression in 65.63%. COX-2 expression was seen in 38.54% samples. Cytoplasmic expression of HuR was significantly associated with increased

COX-2 expression (P < 0.025) as well as with sex (P < 0.040), grade (P < 0.34), and high tumour stage (P < 0.035). There was no significant correlation between HuR (nuclear or cytoplasmic) expression and patient survival. When OSCC cell lines were treated with HuR small inhibitory RNA, expressions of HuR and COX-2 were reduced. Conclusion: Our results suggest that cytoplasmic expression of HuR associates with COX-2 expression, and tissue culture experiments show that HuR can regulate expression of COX-2 in OSCC cells. Further studies are necessary to investigate whether HuR might be a potential target for a molecular tumour therapy. Acknowledgement. This study was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD), Basic research promotion fund (KRF-2008-005-J00803). doi:10.1016/j.ijom.2009.03.509

P37 Survival analysis of 148 patients with stage I and II oral tongue squamous cell carcinoma F. Liang ∗ , T. Nakasone, A. Arasaki, A. Matayoshi, H. Takemoto, H. Sunakawa Department of Oral and Maxillofacial Functional Rehabilitation, Faculty of Medicine, University of the Ryukyus, Nakagami, Okinawa, Japan

Background and Objectives: There are many factors affecting the prognosis of the patients with stage I and II of oral tongue squamous cell carcinoma. This study was to analyse the most important factors affecting the prognosis. Methods: 148 Patients with stage I and II (T1-T2N0M0) SCC of the oral tongue diagnosed between 1986 and 2006 were analysed retrospectively by using the data of long period follow-up. Survival analysis was performed by using the Kaplan-Meier analysis, comparison among groups was analysed using log-rank test. A Cox proportional hazards multivariate analysis was performed to evaluate the influence of covariates on the risk of overall survival (OS). Results: The 5-year OS rate was 88.4%. Univariate analysis showed that primary location recurrence (P = 0.001), occult cervical lymph node metastasis (P = 0.000), and neck dissection (P = 0.019) were factors affecting the prognosis of the patients. On multivariate analysis, the OS was influenced significantly by occult cervical

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